Laminar Air Flow

About Laminar Air Flow

Laminar Air Flow (LAF) Cabinet A Laminar Air Flow cabinet is a closed working bench designed to provide a sterile and particle-free environment for laboratory operations. It is commonly used in microbiology, biotechnology, pharmaceuticals, and research labs to protect sensitive samples from contamination. --- Working Principle The system uses HEPA (High Efficiency Particulate Air) filters to purify air. Room air is drawn in passes through pre-filters then through HEPA filters. HEPA filters remove 99.97% of particles 0.3 microns. The filtered air flows in a uniform laminar stream (unidirectional flow) across the work surface, preventing airborne contaminants from entering the workspace. --- Types of Laminar Air Flow 1. Horizontal LAF air flows horizontally (back front). Provides maximum sample protection. Common in microbiology & pharma labs. 2. Vertical LAF air flows vertically (top bottom). Safer for operator (less chance of air blowing toward the user). Preferred in bioscience and clinical labs. --- Main Components Cabinet Enclosure work area with glass shield. Pre-filters trap large dust particles. HEPA filter removes microbes, fine particles. Blower/Fan pulls and directs airflow. UV Lamp sterilizes the cabinet before use. Fluorescent Lamp illuminates the work surface. Work Table stainless steel, easy to clean. --- Applications Aseptic transfer of microbial cultures. Preparation of sterile media & reagents. Tissue culture work (plant & animal cells). Handling of non-hazardous biological samples. Pharmaceutical quality control testing. --- Advantages Provides sterile, dust-free environment. Protects samples from contamination. Essential for microbiology & cell culture labs.